Human Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ELISA Kit

SKU: orb774955

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor(HBEGF).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HBEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HBEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBEGF in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6.2 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

HB-EGF ELISA Kit, DTR ELISA Kit, DTS ELISA Kit, DTSF ELISA Kit, HEGFL ELISA Kit, Diphtheria Toxin Receptor ELISA Kit, Proheparin-binding EGF-like growth factor ELISA Kit, Diphtheria toxin receptor ELISA Kit

Quick Database Links

UniProt

Q99075