Human Hemoglobin Gamma 2 (HBg2) ELISA Kit

SKU: orb778824

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemoglobin Gamma 2(HBg2).

Research Area

Hematology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HBg2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBg2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HBg2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBg2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, erythrocyte lysates.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 µg/mL
• Sensitivity: 0.64 µg/mL
• Concentration: 100 µg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Gamma-2-globin ELISA Kit

Quick Database Links

UniProt

P69892