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| Catalog Number | orb777935 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hemoglobin Beta(HBb) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hemoglobin Beta(HBb). |
| Tested applications | ELISA |
| Reactivity | Human |
| Range | 7.82-500 pg/mL |
| Assay Time | 2.5h |
| Concentration | 500 pg/mL |
| UniProt ID | P68871 |
| Sensitivity | 2.82 pg/mL |
| Alternative names | HB-B ELISA Kit, HBD ELISA Kit, CD113t-C ELISA Kit, Read more... |
| Note | For research use only |
| Application notes | standard: 500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBb in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma and erythrocyte lysates |
| Expiration Date | Please enquire. |
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Human Hemoglobin Beta ELISA Kit [orb407350]
Human
0.12 ng/mL-500 ng/mL
0.12 ng/mL
24 T, 10 x 96 T, 5 x 96 T, 48 T, 96 T
