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Human Hemoglobin Beta (HBb) ELISA Kit

Catalog Number: orb777935

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb777935
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hemoglobin Beta(HBb) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hemoglobin Beta(HBb).
ReactivityHuman
Concentration500 pg/mL
UniProt IDP68871
Tested applicationsELISA
Application notesstandard: 500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBb in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeCompetitive
Assay Time2.5h
Range7.82-500 pg/mL
Sensitivity2.82 pg/mL
Sample Typesserum, plasma and erythrocyte lysates
Alternative namesHB-B ELISA Kit, HBD ELISA Kit, CD113t-C ELISA Kit,
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NoteFor research use only
Human Hemoglobin Beta (HBb) ELISA Kit

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