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| Catalog Number | orb778378 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 70(HSP70). |
| Tested applications | ELISA |
| Reactivity | Human |
| Range | 3.13-200 ng/mL |
| Assay Time | 3.5h |
| Concentration | 200 ng/mL |
| UniProt ID | P38646 |
| Sensitivity | 1.39 ng/mL |
| Alternative names | DnaK ELISA Kit, 70 Kilodalton Heat Shock Proteins Read more... |
| Note | For research use only |
| Application notes | standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSP-70/HSPA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSP-70/HSPA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSP-70/HSPA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSP-70/HSPA9 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
