Human Growth Hormone R Protein, His Tag

• Catalog Number: orb257505
• Category: Proteins
• Description: Human GHR, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Tyr 264 (Accession # P10912-1).
• Target: Growth Hormone R
• Conjugation: Unconjugated
• Tag: C-10×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 95%
• Protein Sequence: NP_000154.1
• UniProt ID: P10912
• MW: 29.6 kDa
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 29.6 kDa. The protein migrates as 40-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Biological Origin: Human
• Biological Activity: Immobilized Human GH, premium grade (Cat. No. GH1-H4118) at 2 μg/mL (100 μL/well) can bind Human GHR, His Tag (Cat. No. ) with a linear range of 0.078-3 ng/mL (QC tested).
• Expression Region: Ala 27 - Tyr 264
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only
• NCBI: NP_000154.1

References

Nelson, Justin T. et al. Mechanism of immobilized protein A binding to immunoglobulin G on nanosensor array surfaces Anal Chem, 87, 8186-8193 (2015)

PubMed: 26149633

SDS-PAGE analysis of Human Growth Hormone R protein

Immobilized Human GH, premium grade at 2 μg/mL (100 μL/well) can bind Human GHR, His Tag (Cat. No. orb257505) with a linear range of 0.078-3 ng/mL (QC tested).