Human Gremlin 1 (GREM1) ELISA Kit

SKU: orb776964

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gremlin 1(GREM1).

Research Area

Cancer Biology, Musculoskeletal & Connective Tissue Research, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 8000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GREM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GREM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GREM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GREM1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, urine and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 125-8000 pg/mL
• Sensitivity: 55 pg/mL
• Concentration: 8000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

PIG2 ELISA Kit, CKTSF1B1 ELISA Kit, DAND2 ELISA Kit, IHG-2 ELISA Kit, DAND2 ELISA Kit, DRM ELISA Kit, Increased in high glucose protein 2 ELISA Kit, Cell proliferation-inducing gene 2 protein ELISA Kit, Cysteine knot superfamily 1, BMP antagonist 1 ELISA Kit

Quick Database Links

UniProt

O60565