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| Catalog Number | orb779282 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gonadotropin Releasing Hormone(GnRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gonadotropin Releasing Hormone(GnRH). |
| Tested applications | ELISA |
| Reactivity | Human |
| Range | 15.63-1000 pg/mL |
| Assay Time | 2.5h |
| Concentration | 1000 pg/mL |
| UniProt ID | P01148 |
| Sensitivity | 5.21 pg/mL |
| Alternative names | GNRH1 ELISA Kit, GRH ELISA Kit, LNRH ELISA Kit, LH Read more... |
| Note | For research use only |
| Application notes | standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GnRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GnRH in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
- Item 1 of 1
- Item 1 of 1
Human GNRH1 ELISA Kit [orb406143]
Human
2.5 pg/ml-100 pg/ml.
0.625 pg/ml
24 T, 10 x 96 T, 5 x 96 T, 48 T, 96 T
