Human Gonadotropin Releasing Hormone (GnRH) ELISA Kit

SKU: orb779282

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gonadotropin Releasing Hormone(GnRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gonadotropin Releasing Hormone(GnRH).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GnRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GnRH in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 15.63-1000 pg/mL
• Sensitivity: 5.21 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GNRH1 ELISA Kit, GRH ELISA Kit, LNRH ELISA Kit, LHRH ELISA Kit, LH-RH I ELISA Kit, Luliberin ELISA Kit, Luteinizing-Hormone Releasing Hormone ELISA Kit, Progonadoliberin-1 ELISA Kit, Gonadoliberin I ELISA Kit, Gonadorelin ELISA Kit, GnRH-associated peptide 1 ELISA Kit

Quick Database Links

UniProt

P01148