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Catalog Number | orb545735 |
---|---|
Category | Proteins |
Description | Human GM-CSF R alpha, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 320 (Accession # P15509-1). |
Target | GM-CSF R alpha |
Conjugation | Unconjugated |
Tag | C-Fc |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
Purity | 95% |
Protein Sequence | NP_006131.2 |
UniProt ID | P15509 |
MW | 60.9 kDa |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 60.9 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Biological Activity | Loaded Human GM-CSF R alpha, Fc Tag (Cat. No. ) on Protein A Biosensor, can bind Human GM-CSF, premium grade (Cat. No. GMF-H4214) with an affinity constant of 9.21 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | Glu 23 - Gly 320 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_006131.2 |
Loaded Human GM-CSF R alpha, Fc Tag (Cat. No. orb545735) on Protein A Biosensor, can bind Human GM-CSF, premium grade (Cat. No. orb257510) with an affinity constant of 9.21 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 60.6 kDa after removal of the signal peptide.The apparent molecular mass of GM-CSFR-hFc is approximately 70-130 kDa due to glycosylation. | |
Mammalian |