Human Glycoprotein 39, Cartilage (GP39) ELISA Kit

• Catalog Number: orb775855
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein 39, Cartilage(GP39).
• Reactivity: Human
• Concentration: 2000 pg/mL
• UniProt ID: P36222
• Tested applications: ELISA
• Application notes: standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GP39. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GP39. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GP39, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GP39 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 13.1 pg/mL
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Alternative names: CHI3L1 ELISA Kit, CHI3-L1 ELISA Kit, YKL40 ELISA K
• Note: For research use only

References

B Gajewski, I Karlińska, M Domowicz, I Bednarski No Relation Between Cognitive Impairment, Physical Disability and Serum Biomarkers in a Cohort of Progressive Multiple Sclerosis Patients Biomolecules,, (2025)

PubMed:

Applications

ELISA

Reactivity

Human