Human Glutathione S Transferase Omega 1 (GSTo1) ELISA Kit

SKU: orb776829

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Omega 1(GSTo1).

Research Area

Cancer Biology, Gastroenterology & Hepatology, Metabolism Research, Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GSTo1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GSTo1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GSTo1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GSTo1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.056 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

P28 ELISA Kit, GSTTLp28 ELISA Kit, GSTw1 ELISA Kit, SPG-R ELISA Kit, Glutathione-dependent dehydroascorbate reductase ELISA Kit, Monomethylarsonic acid reductase ELISA Kit, MMA(V) reductase ELISA Kit, S-(Phenacyl)glutathione reductase ELISA Kit

Quick Database Links

UniProt

P78417