Human Glutamate Decarboxylase 2, Acid (GAD2) ELISA Kit

SKU: orb775696

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Decarboxylase 2, Acid(GAD2).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GAD2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GAD2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GAD2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GAD2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.79-50 ng/mL
• Sensitivity: 0.28 ng/mL
• Concentration: 50 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GAD65 ELISA Kit, Pancreatic Islets And Brain,65kDa ELISA Kit, 65 kDa glutamic acid decarboxylase ELISA Kit, Glutamate decarboxylase 65 kDa isoform ELISA Kit

Quick Database Links

UniProt

Q05329