Human Glucosidase Beta, Acid (GbA) ELISA Kit

SKU: orb776949

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucosidase Beta, Acid(GbA).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GbA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GbA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GbA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GbA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 12.6 pg/mL
• Concentration: 2000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GBA1 ELISA Kit, GCB ELISA Kit, GLUC ELISA Kit, Imiglucerase ELISA Kit, Alglucerase ELISA Kit, Beta-glucocerebrosidase ELISA Kit, Glucosylceramidase ELISA Kit, β-Glucosidase ELISA Kit, D-Glucosyl-N-Acylsphingosine Glucohydrolase ELISA Kit

Quick Database Links

UniProt

P04062