Human Glucose Regulated Protein 78 (GRP78) ELISA Kit

SKU: orb776803

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 5(HSPA5).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GRP78. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GRP78. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GRP78, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GRP78 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.133 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

MIF2 ELISA Kit, BIP ELISA Kit, GRP78 ELISA Kit, Immunoglobulin heavy chain-binding protein ELISA Kit, Glucose Regulated Protein 78 ELISA Kit, Binding Immunoglobulin Protein ELISA Kit, Endoplasmic reticulum lumenal Ca binding grp78 ELISA Kit

Quick Database Links

UniProt

P11021