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Human Glucagon (GC) ELISA Kit

Catalog Number: orb774946

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb774946
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Glucagon(GC) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glucagon(GC).
ReactivityHuman
Concentration1500 pg/mL
UniProt IDP01275
Tested applicationsELISA
Application notesstandard: 1500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GC in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time2.5h
Range23.44-1500 pg/mL
Sensitivity7.21 pg/mL
Sample Typesserum, plasma and other biological fluids
Alternative namesGCG ELISA Kit, GLP1 ELISA Kit, GLP2 ELISA Kit, GRP
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NoteFor research use only
Human Glucagon (GC) ELISA Kit

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    12.5pg/ml-400pg/ml

    1.0pg/ml

    96 T, 48 T