Human Glial Fibrillary Acidic Protein (GFAP) ELISA Kit

SKU: orb775490

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glial Fibrillary Acidic Protein(GFAP).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GFAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GFAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GFAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GFAP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.058 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Intermediate Filament Protein ELISA Kit

Quick Database Links

UniProt

P14136

References

Włodzimierz Płotek 1, Artur Bekała 2, Jadwiga Łuczak-Wawrzyniak 2, Katarzyna Dudzińska-Rapczewska 2, Małgorzata Gasińska-Błotniak 1, Marcin Cybulski 3, Agnieszka Kubik-Komar 4, Elżbieta Kubera 4, Maciej Wilczak 5, Wojciech Dąbrowski 6 Is general anesthesia neuroinjury-free and safe for gynecological patients' cognition? A comparison of two typical anesthesia schemes based on propofol and sevoflurane Anaesthesiol Intensive Ther, (2025)

PubMed: 40891792

Applications

ELISA

Reactivity

Human