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Catalog Number | orb689462 |
---|---|
Category | Proteins |
Description | Recombinant human GITR Ligand protein with C-terminal His tag |
Reactivity | Human |
Tag | C-6×His Tag |
Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
MW | The protein has a predicted molecular mass of 15.3 kDa after removal of the signal peptide. |
Target | GITR Ligand |
UniProt ID | Q9UNG2 |
Source | Mammalian |
Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
Alternative names | TNFSF18;AITRL;TL6;hGITRL;GITR Ligand Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Human IL1B Protein, hFc Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human IL1B Protein, hFc Tag (orb1173751) can bind Anti-IL1B Neutralizing antibody orb1291211 in a linear range of 0.61–156.25 ng/ml.
ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human IL1R2 Protein, His Tag (orb1290929) can bind Human IL1B Protein, hFc Tag (orb1173751) in a linear range of 3.20–400 ng/ml.
ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human IL1B Protein, hFc Tag (orb1173751) can bind Human IL1RA Protein, mFc Tag (orb2321379) in a linear range of 0.64–80 ng/ml.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 46-53 kDa after removal of the signal peptide. | |
Mammalian |
98.00% | |
47.5 kDa (predicted). Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
98.00% | |
47.5 kDa (predicted). Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
Unconjugated | |
90% | |
20.5 kDa | |
Human GITR Ligand, His Tag (orb612155) is expressed from human 293 cells (HEK293). It contains AA Gln 50 - Ser 177 (Accession # AAH69319.1). |