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Catalog Number | orb2705226 |
---|---|
Category | Proteins |
Description | Human GITR Ligand Protein, Fc Tag, active trimer is expressed from human 293 cells (HEK293). It contains AA Gln 50 - Ser 177 (Accession # AAH69319.1). |
Target | GITR Ligand |
Conjugation | Unconjugated |
Tag | N-Fc |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
MW | 71.2 kDa |
Application notes | This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 71.2 kDa. The protein migrates as 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Expression Region | Gln 50 - Ser 177 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 46-53 kDa after removal of the signal peptide. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 40.6 kDa after removal of the signal peptide. The apparent molecular mass of hFc-mGITRL is approximately 40-55 kDa due to glycosylation. | |
Mammalian |