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Catalog Number | orb257507 |
---|---|
Category | Proteins |
Description | Human GITR Ligand, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gln 50 - Ser 177 (Accession # AAH69319.1). |
Target | GITR Ligand |
Conjugation | Unconjugated |
Tag | N-Fc |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, pH 7.5 |
Purity | 95% |
Protein Sequence | AAH69319.1 |
MW | 41.5 kDa |
Application notes | This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 41.5 kDa. The protein migrates as 45-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Expression Region | Gln 50 - Ser 177 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | AAH69319.1 |
SDS-PAGE analysis of Human GITR Ligand protein
Line graph illustrates about the sensitivity range of Human GITR Ligand protein using different concentrations
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 46-53 kDa after removal of the signal peptide. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 40.6 kDa after removal of the signal peptide. The apparent molecular mass of hFc-mGITRL is approximately 40-55 kDa due to glycosylation. | |
Mammalian |