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Catalog Number | orb18504 |
---|---|
Category | Proteins |
Description | Mouse GITR Ligand, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Thr 47 - Ser 173 (Accession # AAO89011). |
Target | GITR Ligand |
Conjugation | Unconjugated |
Tag | N-Fc |
Reactivity | Mouse |
Form/Appearance | Powder |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
Purity | 95% |
Protein Sequence | AAO89011 |
UniProt ID | Q7TS55 |
RRID | AB_10750094 |
MW | 41.4 kDa |
Application notes | This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 41.4 kDa. The protein migrates as 45 kDa under reducing (R) condition, and 80-100 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Mouse |
Expression Region | Thr 47 - Ser 173 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | AAO89011 |
SDS-PAGE analysis of Mouse GITR Ligand protein
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 46-53 kDa after removal of the signal peptide. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 40.6 kDa after removal of the signal peptide. The apparent molecular mass of hFc-mGITRL is approximately 40-55 kDa due to glycosylation. | |
Mammalian |