Human Ghrelin (GHRL) ELISA Kit

SKU: orb775807

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ghrelin(GHRL) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ghrelin(GHRL).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GHRL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GHRL in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, saliva, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 156.25-10000 pg/mL
• Sensitivity: 49.6 pg/mL
• Concentration: 10000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

MTLRP ELISA Kit, Growth Hormone-Releasing Peptide ELISA Kit

Quick Database Links

UniProt

Q9UBU3

References

Monika Grabia-Lis et al. Development of a novel SPRi carboxymethyl dextran biosensor for sensitive detection of ghrelin in biological samples Talanta, (2025)

PubMed: 41338015

Applications

ELISA

Reactivity

Human