Human GDP Dissociation Inhibitor 1 (GDI1) ELISA Kit

SKU: orb778499

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDP Dissociation Inhibitor 1(GDI1).

Research Area

Neuroscience, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GDI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GDI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GDI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GDI1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.05 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GDIL ELISA Kit, MRX41 ELISA Kit, MRX48 ELISA Kit, OPHN2 ELISA Kit, RABGD1A ELISA Kit, RABGDIA ELISA Kit, XAP-4 ELISA Kit, Guanine Nucleotide Dissociation Inhibitor ELISA Kit, Rab GDP Dissociation Inhibitor Alpha 1 ELISA Kit, Oligophrenin-2 ELISA Kit

Quick Database Links

UniProt

P31150