Human GDF-2 Protein

• Catalog Number: orb735040
• Category: Proteins
• Description: Human Growth and differentiation factor 2 (GDF-2), also known as Bone morphogenetic protein 9 (BMP-9), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. GDF-2 Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG. ALK1 is a signalling receptor for bone morphogenetic protein-9 (BMP-9) in endothelial cells (ECs). BMP-9 bound with high affinity to ALK1 and endoglin, and weakly to the type-I receptor ALK2 and to the BMP type-II receptor (BMPR-II) and activin type-II receptor (ActR-II) in transfected COS cells. Binding of BMP-9 to ALK2 was greatly facilitated when BMPR-II or ActR-II were co-expressed.
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 12.1 kDa
• Target: GDF-2
• Source: Human GDF-2, Tag Free (orb735040) is expressed from human 293 cells (HEK293). It contains AA Ser 320 - Arg 429 (Accession # Q9UK05-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human GDF-2, Tag Free at 1 μg/mL (100 μL/well) can bind Human ALK-1, His Tag with a linear range of 2-16 ng/mL (QC tested).
• Expression Region: Ser 320 - Arg 429
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: ACN, TFA in Water
• Alternative names: GDF-2,GDF2,BMP-9,BMP9
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 12.1 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human GDF-2, Tag Free (Cat. No. orb735040) at 1 μg/mL (100 μL/well) can bind Human ALK-1, His Tag with a linear range of 2-16 ng/mL (QC tested).