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Catalog Number | orb391782 |
---|---|
Category | Proteins |
Description | Recombinant human GC protein is produced by our Mammalian expression system and the target gene encoding Leu17-Leu474 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | GC |
Entrez | 2638 |
UniProt ID | P02774 |
Protein Sequence | LERGRDYEKN KVCKEFSHLG KEDFTSLSLV LYSRKFPSGT FEQVSQLVKE VVSLTEACCA EGADPDCYDT RTSALSAKSC ESNSPFPVHP GTAECCTKEG LERKLCMAAL KHQPQEFPTY VEPTNDEICE AFRKDPKEYA NQFMWEYSTN YGQAPLSLLV SYTKSYLSMV GSCCTSASPT VCFLKERLQL KHLSLLTTLS NRVCSQYAAY GEKKSRLSNL IKLAQKVPTA DLEDVLPLAE DITNILSKCC ESASEDCMAK ELPEHTVKLC DNLSTKNSKF EDCCQEKTAM DVFVCTYFMP AAQLPELPDV ELPTNKDVCD PGNTKVMDKY TFELSRRTHL PEVFLSKVLE PTLKSLGECC DVEDSTTCFN AKGPLLKKEL SSFIDKGQEL CADYSENTFT EYKKKLAERL KAKLPDATPT ELAKLVNKRS DFASNCCSIN SPPLYCDSEI DAELKNILVD HHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Alternative names | Vitamin D-binding protein; DBP; VDB; Gc-globulin; Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Human | |
93.75 pg/ml - 6000 pg/ml | |
50 pg/ml |
Greater than 90% as determined by SDS-PAGE. | |
49.9 kDa | |
E.coli |
Greater than 85% as determined by SDS-PAGE. | |
48.5 kDa | |
Baculovirus |
Greater than 85% as determined by SDS-PAGE. | |
54.5 kDa | |
E.coli |
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