Human Gastric Intrinsic Factor (GIF) ELISA Kit

• Catalog Number: orb777076
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gastric Intrinsic Factor(GIF) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gastric Intrinsic Factor(GIF).
• Tested applications: ELISA
• Reactivity: Human
• Range: 3.13-200 ng/mL
• Assay Time: 2.5h
• Concentration: 200 ng/mL
• UniProt ID: P27352
• Sensitivity: 0.91 ng/mL
• Alternative names: TCN3 ELISA Kit, IF ELISA Kit, IFMH ELISA Kit, INF
• Note: For research use only
• Application notes: standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GIF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GIF in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Serum, plasma and other biological fluids.
• Expiration Date: Please enquire.