Human GAPDH Protein, His Tag

Catalog Number: orb257495

Catalog Number	orb257495
Category	Proteins
Description	Human GAPDH, His Tag is expressed from E. coli cells. It contains AA Gly 2 - Glu 335 (Accession # AAH01601).
Target	GAPDH
Conjugation	Unconjugated
Tag	N-6×His
Reactivity	Human
Form/Appearance	Powder
Buffer/Preservatives	50 mM Tris, 150 mM NaCl, pH 7.5
Purity	95%
Protein Sequence	AAH01601
UniProt ID	P04406
MW	36.9 kDa
Application notes	This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 36.9 kDa. The protein migrates as 37-38 kDa under reducing (R) condition (SDS-PAGE).
Expression System	E. coli
Endotoxins	1.0 EU per μg
Biological Origin	Human
Expression Region	Gly 2 - Glu 335
Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Note	For research use only
NCBI	AAH01601

SDS-PAGE analysis of Human GAPDH protein

Purity:	98.00%
MW:	38 kDa (predicted); 38 kDa (reducing conditions)

Purity:	> 93%, determined by SDS-PAGE
Source:	This protein contains the human GAPDH (P04406) (Met 1-Glu 335) was expressed with a polyhistidine tag at the N-terminus and expressed from E. coli.

Purity:	> 85%, determined by SDS-PAGE
Source:	This protein contains the human GAPDH (P04406) (Met 1-Glu 335) was expressed with a polyhistidine tag at the N-terminus and expressed from E. coli.