Human Galectin 4 (GAL4) ELISA Kit

SKU: orb776756

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 4(GAL4).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GAL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GAL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GAL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GAL4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 62.5-4000 pg/mL
• Sensitivity: 27.4 pg/mL
• Concentration: 4000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

LGALS4 ELISA Kit, Lactose-Binding Lectin 4 ELISA Kit, L-36 Lactose-Binding Protein ELISA Kit, L36LBP ELISA Kit, Lectin,Galactoside-Binding Soluble 4 ELISA Kit

Quick Database Links

UniProt

P56470