Human G Protein Coupled Receptor 172A (GPR172A) ELISA Kit

SKU: orb778885

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Receptor 172A(GPR172A).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPR172A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPR172A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPR172A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPR172A in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, saliva and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.59 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

SLC52A2 ELISA Kit, PAR1 ELISA Kit, GPCR41 ELISA Kit, RFT3 ELISA Kit, Porcine endogenous retrovirus A receptor 1 ELISA Kit, Riboflavin transporter 3 ELISA Kit, Solute carrier family 52, riboflavin transporter, member 2 ELISA Kit