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Item 1 of 2
| Catalog Number | orb257497 |
|---|---|
| Category | Proteins |
| Description | Human G-CSF R, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Pro 621 (Accession # NP_000751.1). |
| Target | G-CSF R |
| Conjugation | Unconjugated |
| Tag | C-6×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| Protein Sequence | NP_000751.1 |
| UniProt ID | Q99062 |
| MW | 67.4 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 67.4 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Immobilized Human G-CSF, Tag Free (Cat. No. GCF-H5214) at 2 μg/mL (100 μL/well) can bind Human G-CSF R, His Tag (Cat. No. ) with a linear range of 0.01-0.313 μg/mL (QC tested). |
| Expression Region | Glu 25 - Pro 621 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_000751.1 |
Ko, Sung Hee et al. Nanofluidic device for continuous multiparameter quality assurance of biologics Nat Nanotechnol, 12, 804-812 (2017)
SDS-PAGE analysis of Human G-CSF R protein
Immobilized ActiveMax® Human G-CSF, Tag Free (Cat. No. orb257496) at 2 μg/mL (100 μL/well) can bind Human G-CSF R, His Tag (Cat. No. orb257497) with a linear range of 0.01-0.313 μg/mL (QC tested).
