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Catalog Number | orb257481 |
---|---|
Category | Proteins |
Description | Human FOLR2, His Tag is expressed from human 293 cells (HEK293). It contains AA Thr 17 - His 228 (Accession # P14207-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 26.5 kDa |
Target | FOLR2 |
UniProt ID | P14207 |
Protein Sequence | NP_000794.3 |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Thr 17 - His 228 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000794.3 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | Tris and Glycine, pH 7.5 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.5 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to N-linked glycosylation which has been verified by PNGase F digestion. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human FOLR2 protein
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 25.4 kDa after removal of the signal peptide. The apparent molecular mass of FOLR2-His is approximately 25-35 kDa due to glycosylation. | |
Mammalian |
Greater than 90.0% as determined by SDS-PAGE. | |
HEK293 Cells |
> 87%, determined by SDS-PAGE | |
This protein contains the human FOLR2 (P14207-1) without the propeptide (Met 1-His 228) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |
> 98%, determined by SDS-PAGE | |
This protein contains the human FOLR2 (P14207-1) without the propeptide (Met 1-His 228) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |