Recombinant Human FLT3LG Protein

SKU: orb3002460

Description

Recombinant Human Fms-like Tyrosine Kinase 3 Ligand is produced by our Mammalian expression system and the target gene encoding Thr27-Pro184 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Activity: ELISA: Immobilized Human FLT3LG (C-6His)(Cat. No.:orb3002460) at 5 μg/ml (100 μl/well) can bind Human FLT3 (C-Fc)(Cat. No.:orb2993431). The EC50 of Human FLT3 (C-Fc)(Cat. No.:orb2993431) is not higher than 10 ng/ml.
• Tag: C-6His
• Molecular Weight: Predicted: 19 KDa. Observed: 24-32 KDa, reducing conditions
• Expression Region: Thr27-Pro184
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 0.01 EU/µg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Fms-Felated Tyrosine Kinase 3 Ligand; Flt3 Ligand; Flt3L; SL Cytokine; FLT3LG

Quick Database Links

UniProt

P49771