Human FK506 Binding Protein 1B (FKBP1B) ELISA Kit

SKU: orb778818

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 1B(FKBP1B).

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FKBP1B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FKBP1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FKBP1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FKBP1B in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.054 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

FKBP12.6 ELISA Kit, FKBP1L ELISA Kit, FKBP9 ELISA Kit, OTK4 ELISA Kit, PKBP1L ELISA Kit, Rotamase ELISA Kit, 12.6 kDa FK506-binding protein ELISA Kit, Immunophilin FKBP12.6 ELISA Kit, Peptidyl-prolyl cis-trans isomerase FKBP1B ELISA Kit

Quick Database Links

UniProt

P68106