Human Fibulin 3 (FBLN3) ELISA Kit

SKU: orb777417

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibulin 3(FBLN3).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FBLN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FBLN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FBLN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FBLN3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, urine and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.64 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

EFEMP1 ELISA Kit, DHRD ELISA Kit, DRAD ELISA Kit, FBNL ELISA Kit, MLVT ELISA Kit, MTLV ELISA Kit, S1-5 ELISA Kit, EGF-Containing Fibulin-Like Extracellular Matrix Protein 1 ELISA Kit, Fibrillin-Like ELISA Kit, Extracellular protein S1-5 ELISA Kit

Quick Database Links

UniProt

Q12805