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Human Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3) ELISA Kit

Catalog Number: orb778797

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb778797
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibronectin Leucine Rich Transmembrane Protein 3(FLRT3).
Tested applicationsELISA
ReactivityHuman
Range1.57-100 ng/mL
Assay Time3.5h
Concentration100 ng/mL
UniProt IDQ9NZU0
Sensitivity0.57 ng/mL
Alternative namesFibronectin-like domain-containing leucine-rich tr
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NoteFor research use only
Application notesstandard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FLRT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FLRT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FLRT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FLRT3 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesTissue homogenates, cell lysates and other biological fluids.
Expiration DatePlease enquire.
Human Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3) ELISA Kit

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