Human Fibroblast Growth Factor Receptor 2 (FGFR2) ELISA Kit

SKU: orb776954

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor Receptor 2(FGFR2).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FGFR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FGFR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FGFR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 12.4 pg/mL
• Concentration: 2000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD332 ELISA Kit, BEK ELISA Kit, BFR1 ELISA Kit, CEK3 ELISA Kit, CFD1 ELISA Kit, ECT1 ELISA Kit, JWS ELISA Kit, K-SAM ELISA Kit, KGFR ELISA Kit, TK14 ELISA Kit, Bacteria-Expressed Kinase,Keratinocyte Growth Factor Receptor ELISA Kit, Craniofacial Dysostosis 1 ELISA Kit, Crouzon Syndrome ELISA Kit

Quick Database Links

UniProt

P21802