Human FGFb

SKU: orb3002475

Description

Recombinant Human Fibroblast Growth Factor 2/Fibroblast Growth Factor Basic is produced by our E.coli expression system and the target gene encoding Pro143-Ser288 is expressed.

Key Properties

• Expression System: E.coli
• Biological Activity: BLI: Loaded Human FGFb (146AA) (Cat#orb3002475) on APS Biosensor, can bind Human GPC3-Fc(Cat#orb2993899) with an affinity constant of 0.6 nM as determined in BLI assay. (Regularly tested). Cell Based Assay: Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 0.3-2.0 ng/ml (QC verified).
• Tag: Tag free
• Molecular Weight: Predicted: 16.3 KDa. Observed: 17 KDa, reducing conditions
• Expression Region: Pro143-Ser288
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
• Endotoxins: < 0.01 EU/µg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl,100mM NaCl,0.02%Tween 80,4%Mannitol,pH 7.5.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2

Quick Database Links

UniProt

P09038