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Catalog Number | orb752937 |
---|---|
Category | Proteins |
Description | FGF19 Mouse Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a 23-216) containing 205 amino acids and having a molecular mass of 23.0 kDa.FGF19 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques. |
Form/Appearance | Sterile Filtered colorless solution. |
Buffer/Preservatives | FGF19 protein (0.5mg/ml) contains 20mM Tris-HCl(pH8.0), 20% glycerol and 0.1M NaCl. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | DGSHMRPLAF SDAGPHVHYG WGDPIRLRHL YTSGPHGLSS CFLRIRADGV VDCARGQSAH SLLEIKAVAL RTVAIKGVHS VRYLCMGADG KMQGLLQYSE EDCAFEEEIR PDGYNVYRSE KHRLPVSLSS AKQRQLYKNR GFLPLSHFLP MLPMVPEEPE DLRGHLESDM FSSPLETDSM DPFGLVTGLE AVRSPSFEKH HHHHH |
Application notes | Cytokines And Growth Factors |
Source | HEK293 cells |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Alternative names | fibroblast growth factor 19, FGF19. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
47.8 kDa | |
E.coli |
> 95% as determined by SDS-PAGE and HPLC. | |
21.8 kDa | |
E.Coli |
Greater than 95% as determined by reducing SDS-PAGE. | |
23.5 KDa | |
Mammalian |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 22.3 kDa after removal of the signal peptide. The apparent molecular mass of FGF19-His is approximately 15-25 kDa due to glycosylation. | |
E.coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 47.6 kDa after removal of the signal peptide. The apparent molecular mass of FGF19-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |