Human FGF19 Protein, Mouse IgG2a Fc Tag

SKU: orb2999602

Description

Fibroblast Growth Factor 19 (FGF19) is a hormone belonging to the FGF family, encoded by the FGF19 gene. It is primarily synthesized and secreted in the ileum in response to bile acid absorption, acting as a ligand for the farnesoid X receptor (FXR). FGF19's core function is to exert negative feedback on hepatic bile acid synthesis by binding to the Fibroblast Growth Factor Receptor 4 (FGFR4)/Klotho-β complex in the liver, thereby inhibiting cholesterol 7α-hydroxylase (CYP7A1). Additionally, it plays a role in glucose and lipid metabolism. Its mouse ortholog is FGF15, and they are often referred to collectively as FGF15/19. Altered FGF19 levels are implicated in chronic bile acid diarrhea and certain metabolic disorders.

Images & Validation

• Application Notes:
  This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 48.3 kDa. The protein migrates as 53-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: FGF19
• Reactivity: Human
• Tag: C-mFc2(mIgG2a)
• Molecular Weight: 48.3 kDa
• Expression Region: Leu 25 - Lys 216
• Purity: 90%
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

FGF19

UniProt

O95750