Human FGF-4

SKU: orb3002445

Description

Recombinant Human Fibroblast Growth Factor 4 is produced by our E.coli expression system and the target gene encoding Ser54-Leu206 is expressed.

Key Properties

• Expression System: E.coli
• Biological Activity: Cell Based Assay: Measured in a cell proliferation assay using MCF7 Human breast cancer cells.The ED50 for this effect is 2-20 ng/ml (QC verified). Human intestinal organoids (ipsc source) were cultured with Activin A(Cat#orb3002480) , BMP-4(Cat#orb3002515) , EGF (Cat#orb3002493) , Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002448), R-spondin 1 (Cat#orb3002497) and FGF-4 (Cat#orb3002445) . The organoids showed good morphology.
• Tag: Tag free
• Molecular Weight: Predicted: 16.9 KDa. Observed: 16 KDa, reducing conditions
• Expression Region: Ser54-Leu206
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 0.01 EU/µg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mMPB，5％Sucrose，4％Mannitol，0.02％Tween80，pH7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Fibroblast growth factor 4; FGF-4; Heparin secretory-transforming protein 1; HST; HST-1; HSTF-1; Heparin-binding growth factor 4; HBGF-4; Transforming protein KS3; FGF4; HST; HSTF1; KS3

Quick Database Links

UniProt

P08620