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Catalog Number | orb425468 |
---|---|
Category | Proteins |
Description | Recombinant of human FGF 18 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | FGF-18 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4. |
Purity | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Protein Sequence | AEENVDFRIH VENQTRARDD VSRKQLRLYQ LYSRTSGKHI QVLGRRISAR GEDGDKYAQL LVETDTFGSQ VRIKGKETEF YLCMNRKGKL VGKPDGTSKE CVFIEKVLEN NYTALMSAKY SGWYVGFTKK GRPRKGPKTR ENQQDVHFMK RYPKGQPELQ KPFKYTTVTK RSRRIRPTHP A |
Application notes | Cytokines And Growth Factors |
Source | Escherichia Coli |
Biological Activity | The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is 2.0×106 units/mg. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized FGF-18 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage | Stability: Lyophilized FGF-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-18 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
Alternative names | Fibroblast growth factor 18, FGF-18, zFGF5, FGF18. |
Note | For research use only |
HPLC, SDS-PAGE | |
Unconjugated | |
> 95% by SDS-PAGE and HPLC analyses | |
21.0 kDa |
Purity as determined by densitometric image analysis is greater than 95%. | |
Escherichia Coli |
98.40% | |
22.3 kDa (predicted); 32 kDa (reducing condition, due to glycosylation) |