Human fFN(Fetal Fibronectin) Microsample ELISA Kit

SKU: orb2999501

Description

Human fFN(Fetal Fibronectin) Microsample ELISA Kit

Research Area

Infectious Disease & Virology, Signal Transduction

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human fFN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human fFN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human fFN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human fFN in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.66 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ED-B; CIG; GFND; EIEE29; FNZ; LETS; GFND2; SMDCF; MSF; FINC; FN; CMT2N