Human Fc Fragment of IgG Low Affinity IIIa Receptor (FcgR3A) ELISA Kit

SKU: orb777703

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fc Fragment Of IgG Low Affinity IIIa Receptor(FcgR3A).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 2000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FcgR3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FcgR3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FcgR3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FcgR3A in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 ng/mL
• Sensitivity: 11.1 ng/mL
• Concentration: 2000 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD16a ELISA Kit, CD16-A ELISA Kit, FCGR3-A ELISA Kit, FCG3 ELISA Kit, FcgRIII ELISA Kit, IGFR3 ELISA Kit, NA ELISA Kit, Neutrophil Antigen ELISA Kit, Low affinity immunoglobulin gamma Fc region receptor III-A ELISA Kit

Quick Database Links

UniProt

P08637