Human Fascin (FSCN) ELISA Kit

SKU: orb776035

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fascin(FSCN).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FSCN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FSCN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FSCN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FSCN in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.137 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

FSCN1 ELISA Kit, SNL ELISA Kit, p55 ELISA Kit, Singed-like protein ELISA Kit, 55 kDa actin-bundling protein ELISA Kit, Fascin homolog 1, Actin-Bundling Protein(Strongylocentrotus Purpuratus) ELISA Kit, Singed,Drosophila,Homolog-Like ELISA Kit

Quick Database Links

UniProt

Q16658