Human Extracellular Signal Regulated Kinase 2 (ERK2) ELISA Kit

• Catalog Number: orb775801
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Extracellular Signal Regulated Kinase 2(ERK2).
• Reactivity: Human
• Concentration: 3000 pg/mL
• UniProt ID: P28482
• Tested applications: ELISA
• Application notes: standard: 3000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ERK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ERK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ERK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ERK2 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 46.88-3000 pg/mL
• Sensitivity: 18.08 pg/mL
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Alternative names: MAPK1 ELISA Kit, P38 ELISA Kit, P40 ELISA Kit, ERK
• Research Area: Cancer Biology, Protein Biochemistry, Signal Trans
• Note: For research use only