Human Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) ELISA Kit

SKU: orb2667805

Description

Human Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) ELISA Kit

Images & Validation

• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EMMPRIN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EMMPRIN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EMMPRIN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EMMPRIN in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Sample Types: serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.13 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD147; BSG; 5F7; M6; OK; TCSF; Basigin(Ok Blood Group); Collagenase stimulatory factor; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor

Quick Database Links

UniProt

P35613