Human Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) ELISA Kit

SKU: orb778942

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Elongation Factor 1 Alpha 1(EEF1a1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EEF1a1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EEF1a1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EEF1a1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EEF1a1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.053 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CCS-3 ELISA Kit, CCS3 ELISA Kit, EEF-1 ELISA Kit, EEF1A ELISA Kit, EF-Tu ELISA Kit, EF1A ELISA Kit, FLJ25721 ELISA Kit, GRAF-1EF ELISA Kit, LENG7 ELISA Kit, PTI1 ELISA Kit, eEF1A-1 ELISA Kit, Leukocyte Receptor Cluster(LRC)Member 7 ELISA Kit, Elongation factor Tu ELISA Kit

Quick Database Links

UniProt

P68104