Human Estrogen ELISA Kit

SKU: orb782203

Description

Human Estrogen ELISA Kit

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Estrogen. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Estrogen. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Estrogen in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 15.625-1000 pg/mL
• Sensitivity: 4.08 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

E ELISA Kit, Estrogen ELISA Kit

Standard curve for orb782203 ELISA kit.

Quick Database Links

Gene Symbol

Estrogen