Human Estradiol (E2) EasyStep ELISA Kit

• Catalog Number: orb1950181
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human E2 antigen, and the Human E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Range: 25-1600 pg/mL
• Assay Time: 1.5h
• Concentration: 1600 pg/mL
• Sensitivity: 25 pg/mL
• Alternative names: 17B-Estradiol; Oestradiol; Beta-Estradiol
• Note: For research use only
• Application notes: standard: 1600 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human E2 antigen, and the Human E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human E2 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Serum
• Expiration Date: Please enquire.