Human ERBB Receptor feedback inhibitor 1 (ERRFI1) ELISA Kit

SKU: orb1173516

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ERBB receptor feedback inhibitor 1(ERRFI1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ERBB receptor feedback inhibitor 1(ERRFI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ERBB receptor feedback inhibitor 1(ERRFI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ERBB receptor feedback inhibitor 1(ERRFI1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ERRFI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ERRFI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ERRFI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ERRFI1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.067 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ERRFI1, GENE 33, MIG 6, MIG6, RALT

Quick Database Links

UniProt

Q9UJM3