Human Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

SKU: orb776097

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epidermal Growth Factor Receptor 2(EGFR2).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGFR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EGFR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EGFR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.223 ng/mL
• Concentration: 40 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD340 ELISA Kit, ErbB2 ELISA Kit, HER-2/neu ELISA Kit, HER2 ELISA Kit, NEU ELISA Kit, MLN 19 ELISA Kit, NGL ELISA Kit, TKR1 ELISA Kit, p185 ELISA Kit, V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2 ELISA Kit, Neuro/Glioblastoma Derived Oncogene Homolog ELISA Kit