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Catalog Number | orb754736 |
---|---|
Category | Proteins |
Description | Recombinant human Ephrin B2 protein |
Tested applications | ELISA, WB |
Reactivity | Human |
Tag | N-terminal His Tag |
Purity | Greater than 95% as determined by SDS-PAGE |
MW | 22.1 kDa |
Formula | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4 |
UniProt ID | P52799 |
Source | E.Coli |
Expression System | 28-229 |
Expression Region | 28-229 |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4 |
Alternative names | EFN B2, EFNB 2, Efnb2, EFNB2_HUMAN, Eph related re Read more... |
Note | For research use only |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
97% | |
57.9 kDa | |
Human EphB4, His Tag (orb257423) is expressed from human 293 cells (HEK293). It contains AA Leu 16 - Ala 539 (Accession # NP_004435.3). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
Human | |
0.625 ng/mL-40 ng/mL | |
0.156 ng/mL |
> 90% by SDS-PAGE. | |
KMP1101, Recombinant Human Ephrin-B2/EFNB2 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ile28-Ala229) of human Ephrin-B2/EFNB2 (Accession #NP_004084.1) fused with a 6×His Tag at the C-terminus. |
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